Biochem. J. (1996) 314
(215–225) (Printed in Great Britain)
Inositol phosphates in the duckweed Spirodela polyrhiza L.
Charles A. BREARLEY* and David E. HANKE
Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.
We have undertaken an analysis of the inositol phosphates
of Spirodela polyrhiza at a developmental stage when massive
accumulation of InsP6 indicates that a large net synthesis is
occurring. We have identified Ins3P, Ins(1,4)P2,
Ins(3,4)P2 and
possibly Ins(4,6)P2, Ins(3,4,6)P3,
Ins(3,4,5,6)P4, Ins(1,3,4,5,6)P5,
D- and/or
L-Ins(1,2,4,5,6)P5 and
InsP6 and revealed the likely
presence of a second InsP3 with chromatographic properties
similar to Ins(1,4,5)P3. The higher inositol phosphates
identified show no obvious direct link to pathways of metabolism of
second messengers purported to operate in higher plants, nor do
they resemble the immediate products of plant phytase action on
InsP6.
Abbreviation used: ABA, abscisic acid; nomenclature: we have used the nomenclature
of [19] to define the enantiomerism of inositol phosphates
described in this text; that is, where the enantiomers are defined
the D-nomenclature is used; where inositol phosphates
are obtained from uncharacterized biological sources they are referred to
as D- and/or L-isomers
and where a racemic mixture of enantiomers is obtained e.g. by acid hydrolysis,
the inositol phosphates are referred to as
D/L-isomers; d, days.
* To whom correspondence should be addressed.
Received 24 August 1995/4 October 1995; accepted 9 October 1995
The Biochemical Society, London © 1996
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